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human u87 glioblastoma cells  (ATCC)


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    ATCC human u87 glioblastoma cells
    Characterization of sEVs and PKH67‐labelled sEVs uptake. The size distribution of sEVs by NTA from the (A) static (collected at D27‐30), (B) orbital shaker (collected at D9–12) and (C) Organoid‐on‐a‐Chip (collected at D27–30) were determined. (D–F) sEVs STEM images from (D) static (at D18–21), (E) orbital shaker (D27–30), (F) Organoid‐on‐a‐Chip (D9–12). (G) The enrichment of sEVs marker Alix and the low abundance of non‐sEV marker Calnexin were detected with Western blotting. (H, I) Uptake of PKH67‐labelled sEVs by <t>U87</t> <t>Glioblastoma</t> cells was demonstrated (scale bar 200 µm). Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01 compared to the control group.
    Human U87 Glioblastoma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 10604 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u87+htb+14/U-87+MG/pmc13644378-80-0-4
    Average 99 stars, based on 10604 article reviews
    human u87 glioblastoma cells - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Molecular and Proteomic Profiling of Human Cerebral Organoid‑Derived Small Extracellular Vesicles"

    Article Title: Molecular and Proteomic Profiling of Human Cerebral Organoid‑Derived Small Extracellular Vesicles

    Journal: Developmental Neurobiology

    doi: 10.1002/dneu.70062

    Characterization of sEVs and PKH67‐labelled sEVs uptake. The size distribution of sEVs by NTA from the (A) static (collected at D27‐30), (B) orbital shaker (collected at D9–12) and (C) Organoid‐on‐a‐Chip (collected at D27–30) were determined. (D–F) sEVs STEM images from (D) static (at D18–21), (E) orbital shaker (D27–30), (F) Organoid‐on‐a‐Chip (D9–12). (G) The enrichment of sEVs marker Alix and the low abundance of non‐sEV marker Calnexin were detected with Western blotting. (H, I) Uptake of PKH67‐labelled sEVs by U87 Glioblastoma cells was demonstrated (scale bar 200 µm). Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01 compared to the control group.
    Figure Legend Snippet: Characterization of sEVs and PKH67‐labelled sEVs uptake. The size distribution of sEVs by NTA from the (A) static (collected at D27‐30), (B) orbital shaker (collected at D9–12) and (C) Organoid‐on‐a‐Chip (collected at D27–30) were determined. (D–F) sEVs STEM images from (D) static (at D18–21), (E) orbital shaker (D27–30), (F) Organoid‐on‐a‐Chip (D9–12). (G) The enrichment of sEVs marker Alix and the low abundance of non‐sEV marker Calnexin were detected with Western blotting. (H, I) Uptake of PKH67‐labelled sEVs by U87 Glioblastoma cells was demonstrated (scale bar 200 µm). Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01 compared to the control group.

    Techniques Used: Marker, Western Blot, Control

    Effects sEVs obtained from static, orbital shaker and Organoid‐on‐a‐Chip conditions on U87 glioblastoma cells. (A) MTT assay for viability of U87 Glioblastoma for static, orbital shaker, and Organoid‐on‐a‐Chip sEVs. (B, C) Representative Ki67/DAPI immunofluorescence images of U87 glioblastoma cells following sEV treatment and quantification of Ki67–DAPI signal overlap using the Manders coefficient. (D, E) Migration of U87 Glioblastoma cells upon sEV treatment was observed by scratch assay up to 48 h. Scale bar 400 µm. (F, G) Diameter of spheres formed on day 2 and day 4 of sEVs treated U87 Glioblastoma by hanging drop sphere formation assay were quantified. Scale bar 200 µm. Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01 compared to the control group.
    Figure Legend Snippet: Effects sEVs obtained from static, orbital shaker and Organoid‐on‐a‐Chip conditions on U87 glioblastoma cells. (A) MTT assay for viability of U87 Glioblastoma for static, orbital shaker, and Organoid‐on‐a‐Chip sEVs. (B, C) Representative Ki67/DAPI immunofluorescence images of U87 glioblastoma cells following sEV treatment and quantification of Ki67–DAPI signal overlap using the Manders coefficient. (D, E) Migration of U87 Glioblastoma cells upon sEV treatment was observed by scratch assay up to 48 h. Scale bar 400 µm. (F, G) Diameter of spheres formed on day 2 and day 4 of sEVs treated U87 Glioblastoma by hanging drop sphere formation assay were quantified. Scale bar 200 µm. Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01 compared to the control group.

    Techniques Used: MTT Assay, Immunofluorescence, Migration, Scratch Assay, Tube Formation Assay, Control

    Related Articles

    Cell Culture:

    Article Title: Combinatorial targeting of glutamine metabolism and lysosomal-based lipid metabolism effectively suppresses glioblastoma.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER L-Tyrosine Sigma-Aldrich Cat #: T8566 L-Valine Sigma-Aldrich Cat #: V0513 Bromophenol blue sodium salt Sigma-Aldrich Cat #: B8026 Methanol Thermo Fisher Scientific Cat #: BPA412P4 Lenti-X Concentrator Clontech Cat #: 631231 TRIzolTM Reagent Invitrogen Cat #:15596018 TWEEN 80 Sigma-Aldrich Cat #:P8074 Critical commercial assays PowerUpTM SYBRTM Green Master Mix Applied Biosystems Cat #: A25778 Qproteome Mitochondria Isolation Kit QIAGEN Cat #: 37612 LysoSensorTM Yellow/Blue dextran Thermo Fisher Scientific Cat #: L22460 DQ-green BSA ThermoFisher Scientific Cat #: D12050 BODIPYTM 493/503 Thermo Fisher Scientific Cat #: D3922 LysoTrackerTM Red DND-99 Thermo Fisher Scientific Cat #: L7528 Low-Density Lipoprotein from Human Plasma, BODIPYTM FL-LDL complex Thermo Fisher Scientific Cat #: L3483 Rhodamine 123 Thermo Fisher Scientific Cat #: R302 CellROXTM Deep Red reagent Thermo Fisher Scientific Cat #: C10422 MitoTrackerTM Green FM Dye Thermo Fisher Scientific Cat #: M7514 Free fatty acid assay Kit (Quantification) Abcam Cat #: ab65341 Cardiolipin assay Kit Sigma-Aldrich Cat #: MAK362 Amplex Red Cholesterol Assay Kit Invitrogen Cat #: A12216 Glutaminase (GLS) Activity Assay Kit (Fluorometric) Abcam Cat #: ab284547 BCA protein assay kit II Abcam Cat #: ab287853 RIPA lysis buffer Thermo Fisher Scientific Cat #: NC9484499 EDTA-free Protease Inhibitor Cocktail Sigma-Aldrich Cat #: 11836170001 Phosphatase inhibitor Sigma-Aldrich Cat #: 4906837001 Hoechst 33342 Solution Thermo Fisher Scientific Cat #: 62249 Glo Lysis buffer Promega Cat #: E266A Promega Renilla-GloTM Luciferase Assay System Thermo Fisher Scientific Cat #: PRE2710 X-tremeGENETM HP DNA Transfection Reagent Sigma-Aldrich Cat #: 6366236001 ECL kit Cytiva Amersham Cat #: RPN2106 iScript cDNA Synthesis Kit Bio-rad Cat #: 170-8891 Deposited data Metabolomics data This paper NMDR: ST003362 Project DOI: https://doi.org/10.21228/M8QJ9B Experimental models: Cell lines GBM patient-derived primary cell, GBM30 The Ohio State University N/A GBM patient-derived primary cell, GBM6 Mayo clinic N/A GBM patient-derived primary cell, GBM26 Mayo clinic N/A Human GBM cell: U251 Sigma-Aldrich Cat #: 09063001; Human GBM cell: U87 Sigma-Aldrich Cat #: 89081402; Human GBM cell: U373 Sigma-Aldrich Cat #: 08061901; Human GBM cell: T98 ATCC Cat #: #CRL-1690TM ATCC; Human GBM cell: LN229 ATCC Cat #: CRL-2611TM; Human embryonal kidney cell: 293FT InvitrogenTM Cat #: R70007 Experimental models: Organisms/strains Athymic NCr-nu/nu, outbred, NCI stock Charles River Lab Strain # 553 (Continued on next page) e3 Cell Reports Medicine 5, 101706, September 17, 2024 OPEN ACCESS .. Authenticated (short tandem repeat profiling) human GBM cell lines, U251 (Male) and U373 (Male) from Sigma, U87 (HTB-14) (Female), T98 (CRL-1690) (Male), LN (CRL-2611) (Female) cells American Type Culture Collection (ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 5% HyClone fetal bovine serum (FBS), and 4 mM glutamine. ..

    Article Title: Rapid discrimination between clinical Clostridioides difficile infection and colonization by quantitative detection of TcdB toxin using a real-time cell analysis system
    Article Snippet: .. Four cell lines, HeLa (CRM-CCL-2), HS27 (CRL-1634), U87 (HTB-14) and Caco-2 (HTB-37) were procured from the American Type Culture Collection (ATCC) and cultured adherently in Dulbecco’s Modified Eagle’s Medium (DMEM). .. The culture medium was supplemented with 1% glutamine (Wuhan Pricella Bi Co., Ltd., Hubei, China), 10% fetal bovine serum (Thermo Fisher Scientific, Inc., Waltham, United States), and 1% penicillin–streptomycin solution (Wuhan Pricella Bio Co., Ltd., Hubei, China) ( ).

    Article Title: Combinatorial targeting of glutamine metabolism and lysosomal-based lipid metabolism effectively suppresses glioblastoma
    Article Snippet: .. Authenticated (short tandem repeat profiling) human GBM cell lines, U251 (Male) and U373 (Male) from Sigma, U87 (HTB-14) (Female), T98 (CRL-1690) (Male), LN (CRL-2611) (Female) cells American Type Culture Collection (ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 5% HyClone fetal bovine serum (FBS), and 4 mM glutamine. ..

    Modification:

    Article Title: Combinatorial targeting of glutamine metabolism and lysosomal-based lipid metabolism effectively suppresses glioblastoma.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER L-Tyrosine Sigma-Aldrich Cat #: T8566 L-Valine Sigma-Aldrich Cat #: V0513 Bromophenol blue sodium salt Sigma-Aldrich Cat #: B8026 Methanol Thermo Fisher Scientific Cat #: BPA412P4 Lenti-X Concentrator Clontech Cat #: 631231 TRIzolTM Reagent Invitrogen Cat #:15596018 TWEEN 80 Sigma-Aldrich Cat #:P8074 Critical commercial assays PowerUpTM SYBRTM Green Master Mix Applied Biosystems Cat #: A25778 Qproteome Mitochondria Isolation Kit QIAGEN Cat #: 37612 LysoSensorTM Yellow/Blue dextran Thermo Fisher Scientific Cat #: L22460 DQ-green BSA ThermoFisher Scientific Cat #: D12050 BODIPYTM 493/503 Thermo Fisher Scientific Cat #: D3922 LysoTrackerTM Red DND-99 Thermo Fisher Scientific Cat #: L7528 Low-Density Lipoprotein from Human Plasma, BODIPYTM FL-LDL complex Thermo Fisher Scientific Cat #: L3483 Rhodamine 123 Thermo Fisher Scientific Cat #: R302 CellROXTM Deep Red reagent Thermo Fisher Scientific Cat #: C10422 MitoTrackerTM Green FM Dye Thermo Fisher Scientific Cat #: M7514 Free fatty acid assay Kit (Quantification) Abcam Cat #: ab65341 Cardiolipin assay Kit Sigma-Aldrich Cat #: MAK362 Amplex Red Cholesterol Assay Kit Invitrogen Cat #: A12216 Glutaminase (GLS) Activity Assay Kit (Fluorometric) Abcam Cat #: ab284547 BCA protein assay kit II Abcam Cat #: ab287853 RIPA lysis buffer Thermo Fisher Scientific Cat #: NC9484499 EDTA-free Protease Inhibitor Cocktail Sigma-Aldrich Cat #: 11836170001 Phosphatase inhibitor Sigma-Aldrich Cat #: 4906837001 Hoechst 33342 Solution Thermo Fisher Scientific Cat #: 62249 Glo Lysis buffer Promega Cat #: E266A Promega Renilla-GloTM Luciferase Assay System Thermo Fisher Scientific Cat #: PRE2710 X-tremeGENETM HP DNA Transfection Reagent Sigma-Aldrich Cat #: 6366236001 ECL kit Cytiva Amersham Cat #: RPN2106 iScript cDNA Synthesis Kit Bio-rad Cat #: 170-8891 Deposited data Metabolomics data This paper NMDR: ST003362 Project DOI: https://doi.org/10.21228/M8QJ9B Experimental models: Cell lines GBM patient-derived primary cell, GBM30 The Ohio State University N/A GBM patient-derived primary cell, GBM6 Mayo clinic N/A GBM patient-derived primary cell, GBM26 Mayo clinic N/A Human GBM cell: U251 Sigma-Aldrich Cat #: 09063001; Human GBM cell: U87 Sigma-Aldrich Cat #: 89081402; Human GBM cell: U373 Sigma-Aldrich Cat #: 08061901; Human GBM cell: T98 ATCC Cat #: #CRL-1690TM ATCC; Human GBM cell: LN229 ATCC Cat #: CRL-2611TM; Human embryonal kidney cell: 293FT InvitrogenTM Cat #: R70007 Experimental models: Organisms/strains Athymic NCr-nu/nu, outbred, NCI stock Charles River Lab Strain # 553 (Continued on next page) e3 Cell Reports Medicine 5, 101706, September 17, 2024 OPEN ACCESS .. Authenticated (short tandem repeat profiling) human GBM cell lines, U251 (Male) and U373 (Male) from Sigma, U87 (HTB-14) (Female), T98 (CRL-1690) (Male), LN (CRL-2611) (Female) cells American Type Culture Collection (ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 5% HyClone fetal bovine serum (FBS), and 4 mM glutamine. ..

    Article Title: Rapid discrimination between clinical Clostridioides difficile infection and colonization by quantitative detection of TcdB toxin using a real-time cell analysis system
    Article Snippet: .. Four cell lines, HeLa (CRM-CCL-2), HS27 (CRL-1634), U87 (HTB-14) and Caco-2 (HTB-37) were procured from the American Type Culture Collection (ATCC) and cultured adherently in Dulbecco’s Modified Eagle’s Medium (DMEM). .. The culture medium was supplemented with 1% glutamine (Wuhan Pricella Bi Co., Ltd., Hubei, China), 10% fetal bovine serum (Thermo Fisher Scientific, Inc., Waltham, United States), and 1% penicillin–streptomycin solution (Wuhan Pricella Bio Co., Ltd., Hubei, China) ( ).

    Article Title: Combinatorial targeting of glutamine metabolism and lysosomal-based lipid metabolism effectively suppresses glioblastoma
    Article Snippet: .. Authenticated (short tandem repeat profiling) human GBM cell lines, U251 (Male) and U373 (Male) from Sigma, U87 (HTB-14) (Female), T98 (CRL-1690) (Male), LN (CRL-2611) (Female) cells American Type Culture Collection (ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 5% HyClone fetal bovine serum (FBS), and 4 mM glutamine. ..



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    Image Search Results


    Characterization of sEVs and PKH67‐labelled sEVs uptake. The size distribution of sEVs by NTA from the (A) static (collected at D27‐30), (B) orbital shaker (collected at D9–12) and (C) Organoid‐on‐a‐Chip (collected at D27–30) were determined. (D–F) sEVs STEM images from (D) static (at D18–21), (E) orbital shaker (D27–30), (F) Organoid‐on‐a‐Chip (D9–12). (G) The enrichment of sEVs marker Alix and the low abundance of non‐sEV marker Calnexin were detected with Western blotting. (H, I) Uptake of PKH67‐labelled sEVs by U87 Glioblastoma cells was demonstrated (scale bar 200 µm). Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01 compared to the control group.

    Journal: Developmental Neurobiology

    Article Title: Molecular and Proteomic Profiling of Human Cerebral Organoid‑Derived Small Extracellular Vesicles

    doi: 10.1002/dneu.70062

    Figure Lengend Snippet: Characterization of sEVs and PKH67‐labelled sEVs uptake. The size distribution of sEVs by NTA from the (A) static (collected at D27‐30), (B) orbital shaker (collected at D9–12) and (C) Organoid‐on‐a‐Chip (collected at D27–30) were determined. (D–F) sEVs STEM images from (D) static (at D18–21), (E) orbital shaker (D27–30), (F) Organoid‐on‐a‐Chip (D9–12). (G) The enrichment of sEVs marker Alix and the low abundance of non‐sEV marker Calnexin were detected with Western blotting. (H, I) Uptake of PKH67‐labelled sEVs by U87 Glioblastoma cells was demonstrated (scale bar 200 µm). Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01 compared to the control group.

    Article Snippet: Human U87 glioblastoma cells (American Type Culture Collection) were cultured in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin.

    Techniques: Marker, Western Blot, Control

    Effects sEVs obtained from static, orbital shaker and Organoid‐on‐a‐Chip conditions on U87 glioblastoma cells. (A) MTT assay for viability of U87 Glioblastoma for static, orbital shaker, and Organoid‐on‐a‐Chip sEVs. (B, C) Representative Ki67/DAPI immunofluorescence images of U87 glioblastoma cells following sEV treatment and quantification of Ki67–DAPI signal overlap using the Manders coefficient. (D, E) Migration of U87 Glioblastoma cells upon sEV treatment was observed by scratch assay up to 48 h. Scale bar 400 µm. (F, G) Diameter of spheres formed on day 2 and day 4 of sEVs treated U87 Glioblastoma by hanging drop sphere formation assay were quantified. Scale bar 200 µm. Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01 compared to the control group.

    Journal: Developmental Neurobiology

    Article Title: Molecular and Proteomic Profiling of Human Cerebral Organoid‑Derived Small Extracellular Vesicles

    doi: 10.1002/dneu.70062

    Figure Lengend Snippet: Effects sEVs obtained from static, orbital shaker and Organoid‐on‐a‐Chip conditions on U87 glioblastoma cells. (A) MTT assay for viability of U87 Glioblastoma for static, orbital shaker, and Organoid‐on‐a‐Chip sEVs. (B, C) Representative Ki67/DAPI immunofluorescence images of U87 glioblastoma cells following sEV treatment and quantification of Ki67–DAPI signal overlap using the Manders coefficient. (D, E) Migration of U87 Glioblastoma cells upon sEV treatment was observed by scratch assay up to 48 h. Scale bar 400 µm. (F, G) Diameter of spheres formed on day 2 and day 4 of sEVs treated U87 Glioblastoma by hanging drop sphere formation assay were quantified. Scale bar 200 µm. Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01 compared to the control group.

    Article Snippet: Human U87 glioblastoma cells (American Type Culture Collection) were cultured in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin.

    Techniques: MTT Assay, Immunofluorescence, Migration, Scratch Assay, Tube Formation Assay, Control